Anti-Human EGFR Recombinant Antibody (Zalutumumab) (CAT#: TAB-040)

Recombinant monoclonal antibody to EGFR. Zalutumumab (proposed trade name HuMax-EGFr) is a fully human IgG1 monoclonal antibody (mAb) directed towards the epidermal growth factor receptor (EGFr). the Netherlands. Specifically, zalutumumab is designed for the treatment of squamous cell carcinoma of the head and neck (SCCHN), a type of cancer. The current treatment for SCCHN includes tyrosine kinase inhibitors and human monoclonal antibodiess. In addition, murine mAbs were tested successfully in mice, but caused an immune response in humans and were thus determined as ineffective in therapeutics.

Host: Human

Isotype: IgG1 - kappa

Applications: ELISA, FC, IP, FuncS, IF, Neut, ICC


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  • Published Data
  • Tested Data
  • Gene Expression
  • Datasheet
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  • COA

Figure 1 Development of a panel of EGFR-specific mAbs with distinct functionalities.

A, Binding of zalutumumab, zalu-N297Q, mAb 018, and mAb 018-N297Q to cell-associated EGFR on A431 cells analyzed by flow cytometry; data were analyzed using a four-parameter logistic curve fit. Shown is the mean fluorescence intensity (MFI). B, The capacity of the different mAbs to induce ADCC analyzed in a 51Cr-release assay using human PBMCs as effector cells and A431 cells as target cells. Data shown are percentages specific lysis, calculated as described in Materials and Methods. C, mAb-induced inhibition of EGFR phosphorylation in A431 cells upon stimulation with EGF measured in an EGFR phosphorylation inhibition assay. Data shown are time-resolved fluorescences (TRF). D, Inhibition of A431 cell proliferation determined in a proliferation inhibition assay using Alamar Blue staining. Each data point represents mean ± SEM of triplicates, and experiments shown are representative of at least two independent experiments.

Overdijk, M. B., Verploegen, S., van den Brakel, J. H., van Bueren, J. J. L., Vink, T., van de Winkel, J. G., ... & Bleeker, W. K. (2011). Epidermal growth factor receptor (EGFR) antibody-induced antibody-dependent cellular cytotoxicity plays a prominent role in inhibiting tumorigenesis, even of tumor cells insensitive to EGFR signaling inhibition. The Journal of Immunology, 1003926.

Figure 2 Zalutumumab and mAb 018 are functional in an ADCC assay with murine effector cells.

The capacity of the different mAbs to induce ADCC was analyzed in a 51Cr-release assay using mouse bone marrow-derived macrophages as effector cells, and A431 cells as target cells. mAbs were tested at a concentration of 10 μg/ml with six replicates. Data represent mean ± SEM of three independent experiments.

Overdijk, M. B., Verploegen, S., van den Brakel, J. H., van Bueren, J. J. L., Vink, T., van de Winkel, J. G., ... & Bleeker, W. K. (2011). Epidermal growth factor receptor (EGFR) antibody-induced antibody-dependent cellular cytotoxicity plays a prominent role in inhibiting tumorigenesis, even of tumor cells insensitive to EGFR signaling inhibition. The Journal of Immunology, 1003926.

Figure 3 Both zalutumumab and mAb 018 are capable of inhibiting growth of tumor cells expressing mutant KRAS.

Eight mice per group were inoculated s.c. with 1 × 106 A431-KRAS4bG12V cells, which are insensitive for EGFR signaling inhibition. A, Single treatment at day 0 with 5 mg/kg mAb. B, Single treatment at day 0, at indicated dose levels. Data shown are mean tumor volumes ± SEM.

Overdijk, M. B., Verploegen, S., van den Brakel, J. H., van Bueren, J. J. L., Vink, T., van de Winkel, J. G., ... & Bleeker, W. K. (2011). Epidermal growth factor receptor (EGFR) antibody-induced antibody-dependent cellular cytotoxicity plays a prominent role in inhibiting tumorigenesis, even of tumor cells insensitive to EGFR signaling inhibition. The Journal of Immunology, 1003926.

Figure 4 Histology and immunohistochemistry of biopsies from zalutumumab-injected skin treated with HuMab-10F8 or untreated.

Data shown are representative sections of skin biopsies of one subject for part III of the study. Biopsies were taken at day 14 after two injections with 100 µg of zalutumumab with [A, C, E, G, I, K] or without HuMab-10F8 [B, D, F, H, J, L]. A, B: HE staining; C, D: staining for EGFR; E–H: staining for macrophages and I–L: staining for neutrophils. Extensive influx of inflammatory cells in the vicinity of the hair follicle with histological signs of folliculitis was observed in zalutumumab-injected skin (right panels), whereas in the zalutumumab-injected skin treated with HuMab-10F8 (left panels) inflammatory cells were virtually absent and sebaceous glands and hair follicles showed normal histological features. Inflammatory infiltrate in the zalutumumab-injected skin was mainly composed of neutrophils (neutrophil elastase positive cells, J, L) and to a lesser extent of macrophages; (CD68 positive cells F, H). Scale bars are 100 µm (A–F, I, J) and 20 µm (G, H, K, L). G, H, K and L are magnifications of the squared areas indicated in E, F, I and J.

Bangsgaard, N., Houtkamp, M., Schuurhuis, D. H., Parren, P. W., Baadsgaard, O., Niessen, H. W., & Skov, L. (2012). Neutralization of IL-8 prevents the induction of dermatologic adverse events associated with the inhibition of epidermal growth factor receptor. PLoS One, 7(6), e39706.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG1 - kappa
  • Specificity
  • Tested positive against native human antigen.
  • Species Reactivity
  • Human
  • Applications
  • ELISA, FC, IP, FuncS, IF, Neut, ICC
  • Trade name
  • humax-egfr
  • CAS
  • 667901-13-5
  • Generic Name
  • Zalutumumab
  • MW
  • 148 kDa
  • Related Disease
  • Head and neck cancers

Product Property

  • Purity
  • >95.0% as determined by analysis by SDS-PAGE.
  • Storage
  • Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The EGFR antibody has been reported in applications of FC, ADCC, Inhib, IHC.

Target

  • Alternative Names
  • Zalutumumab;humax-egfr;667901-13-5;HuMax-EGFR;2F8;EGFR;epidermal growth factor receptor;epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB;ERBB1;erythroblastic leukemia viral (v erb b) oncogene homolog

Related Resources

  • Biosimilar Overview
  • Related Diseases
  • Related Signaling Pathways
Please click here for more details about the introduction to mechanism of action, clinical projects and approved drugs of Zalutumumab.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Zalutumumab"

Afuco™ Anti-EGFR ADCC Recombinant Antibody (Zalutumumab), ADCC Enhanced
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to EGFR. Zalutumumab (proposed trade name HuMax-EGFr) is a fully human IgG1 monoclonal antibody (mAb) directed towards the epidermal growth factor receptor (EGFr).

See other products for "EGFR"

Single-domain Antibody

CAT Product Name Application Type
NABG-056 Recombinant Anti-Mouse Egfr VHH Single Domain Antibody ELISA, IHC, FC, FuncS Llama VHH
HPAB-AP881-YC Recombinant Llama Anti-EGFR Single Domain Antibody (VHH122) ELISA, FC, IP, FuncS Llama VHH
HPAB-AP882-YC Recombinant Llama Anti-EGFR Single Domain Antibody (VHH205) ELISA Llama VHH
HPAB-AP883-YC Recombinant Llama Anti-EGFR Single Domain Antibody (VHH03) ELISA Llama VHH
HPAB-AP884-YC Recombinant Llama Anti-EGFR Single Domain Antibody (VHH139) ELISA Llama VHH

Human Antibody

CAT Product Name Application Type
TAB-119 Anti-Human EGFR Recombinant Antibody (Necitumumab) FC, IP, ELISA, Neut, FuncS, IF, WB IgG1 - kappa
TAB-321MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (DX 1-4) SPR Human antibody
TAB-322MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (DX 1-6) SPR Human antibody
TAB-323MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (DX 1-10) SPR Human antibody
PABX-053-F (E) Recombinant Human Anti-EGFR Antibody Fab Fragment (DL11) WB, ELISA, FuncS Fab

Humanized Antibody

CAT Product Name Application Type
TAB-753 Anti-EGFR Recombinant Antibody (Imgatuzumab) Neut, ELISA, IF, IP, FuncS, FC, WB IgG1 - kappa
TAB-274MZ Human Anti-EGFR Recombinant Antibody (TAB-274MZ) FC Humanized IgG
TAB-297MZ Anti-Human EGFR Recombinant Antibody (H225) ELISA, WB Humanized antibody
TAB-274MZ-S(P) Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P)) FC Humanized scFv
TAB-274MZ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-274MZ-F(E)) FC Humanized Fab

Recombinant Antibody

CAT Product Name Application Type
PABW-171 Mouse Anti-EGFR Recombinant Antibody (PABW-171) WB Mouse IgG
MOB-0094MZ Recombinant Mouse Anti-EGFR Antibody (clone FGF-R2) ELISA, IHC-P, FC Mouse antibody
TAB-477CQ Human Anti-EGFR Recombinant Antibody (clone Laprituximab) ELISA, IHC, FC, IP, IF, WB Chimeric (mouse/human) IgG1, κ
MOB-052LC Mouse Anti-EGFR Recombinant Antibody (clone 7H7) WB, IP Mouse IgG
MOB-053LC Mouse Anti-EGFR Recombinant Antibody (clone B11) ELISA, IF, IP, WB Mouse IgG

Chimeric Antibody

CAT Product Name Application Type
TAB-272MZ Human Anti-EGFR Recombinant Antibody (TAB-272MZ) FuncS Chimeric (mouse/human) IgG
TAB-302MZ Human Anti-EGFR Recombinant Antibody (TAB-302MZ) ELISA Chimeric (mouse/human) IgG1
TAB-272MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-272MZ-S(P)) FuncS Mouse scFv
TAB-302MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-302MZ-S(P)) ELISA Mouse scFv
TAB-302MZ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-302MZ-F(E)) ELISA Chimeric (mouse/human) Fab

Mouse Antibody

CAT Product Name Application Type
TAB-304MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-304MZ-F(E)) ELISA Mouse Fab
TAB-305MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-305MZ-F(E)) ELISA Mouse Fab
TAB-306MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-306MZ-F(E)) ELISA Mouse Fab
TAB-307MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-307MZ-F(E)) ELISA, FC Mouse Fab
TAB-310MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-310MZ-F(E)) ELISA Mouse Fab

Fc Glycosylation

CAT Product Name Application Type
Gly-055LC Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)
Gly-144LC Recombinant Anti-Human EGFR Antibody (Fc glycosylation) ELISA Humanized antibody

High-mannose Glycoform

CAT Product Name Application Type
Gly-055LC-1 Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT Product Name Application Type
Gly-167LC Recombinant Anti-Human EGFR Antibody (Non-glycosylated) ELISA Human antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0183MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody
BRD-0668MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC773 A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer FCM
MHC-LC4545 PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer FCM
MHC-YF409 A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer FCM

Neutralizing Antibody

CAT Product Name Application Type
NEUT-722CQ Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011) Neut Rabbit IgG
NEUT-723CQ Mouse Anti-EGFR Recombinant Antibody (clone CBL931) WB, IP, IHC, ICC, Neut Mouse IgG1
NEUT-724CQ Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J) IF, FC, WB, IP, Neut Rabbit IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1101 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB) IHC-P Rabbit IgG
MOR-4520 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH28DS) IF, ICC, FC Rabbit IgG
MOR-4675 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS) WB, IF, ICC, FC Rabbit IgG
MOR-4676 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH190DS) WB, IF, ICC, FC Rabbit IgG
MOR-0033-FY Rabbit Anti-EGFR Recombinant Antibody (clone AFY0004) ICC, IHC, WB Rabbit IgG

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0010-YC-F(E) Mouse Anti-EGFR Recombinant Antibody (clone L211C); Fab Fragment ELISA, FC Mouse Fab
HPAB-0011-YC-F(E) Human Anti-EGFR Recombinant Antibody (clone Pep 1); Fab Fragment FC Human Fab
HPAB-0012-YC-F(E) Human Anti-EGFR Recombinant Antibody (clone Pep 2); Fab Fragment FC Human Fab
HPAB-0013-YC-F(E) Human Anti-EGFR Recombinant Antibody (clone Pep 3); Fab Fragment FC Human Fab
HPAB-0014-YC-F(E) Human Anti-EGFR Recombinant Antibody (clone Pep 4); Fab Fragment FC Human Fab

scFv Fragment Antibody

CAT Product Name Application Type
FAMAB-0094-YC-S(P) Human Anti-EGFR Recombinant Antibody (clone F4); scFv Fragment ELISA, WB, FC Human scFv
HPAB-S0020-YC-S(P) Human Anti-EGFR Recombinant Antibody (clone E6.2); scFv Fragment ELISA, Inhib Human scFv
HPAB-S0021-YC-S(P) Human Anti-EGFR Recombinant Antibody (clone E6.3); scFv Fragment ELISA, Inhib Human scFv
HPAB-S0022-YC-S(P) Human Anti-EGFR Recombinant Antibody (clone E7.6.3); scFv Fragment ELISA, Inhib, FuncS Human scFv
HPAB-2098-FY-S(P) Mouse Anti-EGFR Recombinant Antibody (clone Mint5); scFv Fragment ICC, Inhib Mouse scFv

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-710 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Nimotuzumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut, IF ADCC enhanced antibody
AFC-TAB-753 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Imgatuzumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody
AFC-TAB-H35 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Futuximab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC, IP ADCC enhanced antibody
AFC-TAB-H49 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Modotuximab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC, IP ADCC enhanced antibody
AFC-TAB-020 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Panitumumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut, IF ADCC enhanced antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
WBWestern Blot

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