Human Anti-FN1 Recombinant Antibody (clone L19)
CAT#: HPAB-S0038-YC
Provided is an antibody with sub-nanomolar affinity specific for a characteristic epitope of the ED-B domain of fibronectin, a marker of angiogenesis.
Gene Expression
Published Data
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_B_9_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_9_3.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_9_4.jpg
Normal Tissue
Figure 4 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_5_6.jpg
Normal Tissue
Figure 5 Placenta
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Trophoblastic cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_1_7.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/126/2077_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (ASC TERT1, HSkMC, hTERT-HME1, hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115414-FN1
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IHC
Figure 1 Immunohistochemical analysis of the distribution of B-FN in human and mouse grafted tumor models.
Blood vessels of F9 tumors are strongly stained by scFv(L19) in vitro using immunohistochemical techniques In all these tumors, L19 stained vascular structures.
Tarli, L., Balza, E., Viti, F., Borsi, L., Castellani, P., Berndorff, D., ... & Zardi, L. (1999). A high-affinity human antibody that targets tumoral blood vessels. Blood, The Journal of the American Society of Hematology, 94(1), 192-198.
IHC
Figure 2 B-FN accumulates around the vessels of exponentially growing tumors but not on vessels of quiescent tumors.
Cryostat sections of the SKMEL-28 xenograft in the exponentially growing phase (A and C) and in the quiescent phase (B and D). Sections shown in (A) and (B) were double stained using the antibody fragment L19 to B-FN (red staining) and the MoAb KI67 specific for proliferating cells (brown staining). Sections shown in (C) and (D) were double stained using the antibody fragment L19 to BFN (red staining) and a rat MoAb recognizing murine CD31, a marker of endothelial cells (brown staining).
Tarli, L., Balza, E., Viti, F., Borsi, L., Castellani, P., Berndorff, D., ... & Zardi, L. (1999). A high-affinity human antibody that targets tumoral blood vessels. Blood, The Journal of the American Society of Hematology, 94(1), 192-198.
Figure 3 Tumor-targeting performance of recombinant antibody fragments.
ScFv(L19) is rapidly eliminated from blood through the kidneys, with a typical biphasic profile.
Tarli, L., Balza, E., Viti, F., Borsi, L., Castellani, P., Berndorff, D., ... & Zardi, L. (1999). A high-affinity human antibody that targets tumoral blood vessels. Blood, The Journal of the American Society of Hematology, 94(1), 192-198.
Figure 4 Microautoradiography of an F9 teratocarcinoma dissected from a nude mouse, after injection of radiolabeled L19.
Two different magnifications of a microautoradiography of a F9 mouse teratocarcinoma. The radiolabeled L19 accumulates around vascular structures within the F9 mouse tumor but not in the surrounding normal mouse tissue (A). At higher magnification (B) within the lumens of some vessels, the red blood cells are visible (arrow).
Tarli, L., Balza, E., Viti, F., Borsi, L., Castellani, P., Berndorff, D., ... & Zardi, L. (1999). A high-affinity human antibody that targets tumoral blood vessels. Blood, The Journal of the American Society of Hematology, 94(1), 192-198.
Figure 5 Dose-response dependence of the tumor-targeting properties of scFv(L19).
Plot of the percent of injected dose per gram of tumor (%ID/g) obtained with different doses of radiolabeled L19. The tumor targeting performance of the antibody is essentially constant over this concentration range.
Tarli, L., Balza, E., Viti, F., Borsi, L., Castellani, P., Berndorff, D., ... & Zardi, L. (1999). A high-affinity human antibody that targets tumoral blood vessels. Blood, The Journal of the American Society of Hematology, 94(1), 192-198.
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Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human IgG
- Specificity
- Human FN1
- Species Reactivity
- Human
- Clone
- L19
- Applications
- ELISA, IF, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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(Creative Biolabs Cat# HPAB-S0038-YC, RRID: AB_3111449)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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