Human Anti-FN1 Recombinant Antibody (clone L19) (CAT#: HPAB-S0038-YC)

Figure 1 Immunohistochemical analysis of the distribution of B-FN in human and mouse grafted tumor models.

Blood vessels of F9 tumors are strongly stained by scFv(L19) in vitro using immunohistochemical techniques In all these tumors, L19 stained vascular structures.

Tarli, L., Balza, E., Viti, F., Borsi, L., Castellani, P., Berndorff, D., ... & Zardi, L. (1999). A high-affinity human antibody that targets tumoral blood vessels. Blood, The Journal of the American Society of Hematology, 94(1), 192-198.

Figure 2 B-FN accumulates around the vessels of exponentially growing tumors but not on vessels of quiescent tumors.

Cryostat sections of the SKMEL-28 xenograft in the exponentially growing phase (A and C) and in the quiescent phase (B and D). Sections shown in (A) and (B) were double stained using the antibody fragment L19 to B-FN (red staining) and the MoAb KI67 specific for proliferating cells (brown staining). Sections shown in (C) and (D) were double stained using the antibody fragment L19 to BFN (red staining) and a rat MoAb recognizing murine CD31, a marker of endothelial cells (brown staining).

Tarli, L., Balza, E., Viti, F., Borsi, L., Castellani, P., Berndorff, D., ... & Zardi, L. (1999). A high-affinity human antibody that targets tumoral blood vessels. Blood, The Journal of the American Society of Hematology, 94(1), 192-198.

Figure 3 Tumor-targeting performance of recombinant antibody fragments.

ScFv(L19) is rapidly eliminated from blood through the kidneys, with a typical biphasic profile.

Tarli, L., Balza, E., Viti, F., Borsi, L., Castellani, P., Berndorff, D., ... & Zardi, L. (1999). A high-affinity human antibody that targets tumoral blood vessels. Blood, The Journal of the American Society of Hematology, 94(1), 192-198.

Figure 4 Microautoradiography of an F9 teratocarcinoma dissected from a nude mouse, after injection of radiolabeled L19.

Two different magnifications of a microautoradiography of a F9 mouse teratocarcinoma. The radiolabeled L19 accumulates around vascular structures within the F9 mouse tumor but not in the surrounding normal mouse tissue (A). At higher magnification (B) within the lumens of some vessels, the red blood cells are visible (arrow).

Tarli, L., Balza, E., Viti, F., Borsi, L., Castellani, P., Berndorff, D., ... & Zardi, L. (1999). A high-affinity human antibody that targets tumoral blood vessels. Blood, The Journal of the American Society of Hematology, 94(1), 192-198.

Figure 5 Dose-response dependence of the tumor-targeting properties of scFv(L19).

Plot of the percent of injected dose per gram of tumor (%ID/g) obtained with different doses of radiolabeled L19. The tumor targeting performance of the antibody is essentially constant over this concentration range.

Tarli, L., Balza, E., Viti, F., Borsi, L., Castellani, P., Berndorff, D., ... & Zardi, L. (1999). A high-affinity human antibody that targets tumoral blood vessels. Blood, The Journal of the American Society of Hematology, 94(1), 192-198.