Recombinant Rabbit Anti-AXL Antibody (clone R06-5B2)
CAT#: VS3-FY127
This product is a recombinant rabbit antibody that recognizes human and rat AXL protein. It can detect a band of approximately 98 kDa (observedd molecular weight: 138 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, actin filaments & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75217/1899_C1_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human thyroid gland
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32501/ihc_selected.jpg
Normal Tissue
Figure 3 Skeletal muscle
Myocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_B_1_6.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37422/82809_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (fHDF/TERT166, U-251 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167601-AXL
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WB
Figure 1. Recombinant Rabbit Anti-AXL Antibody (clone R06-5B2) in WB.
Western blot analysis of AXLRabbit mAb from HeLa lysates using AXL Antibody.
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Specifications
- Immunogen
- Recombinant protein of human Axl
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat AXL
- Species Reactivity
- Human, Rat
- Clone
- R06-5B2
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 98 kDa; Observed MW: 138 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
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Related Diseases
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1087CL | Human Anti-AXL Recombinant Antibody (TAB-1087CL) | ADCC | Chimeric (mouse/human) IgG1, κ |
| TAB-1088CL | Human Anti-AXL Recombinant Antibody (TAB-1088CL) | ADCC | Chimeric (mouse/human) IgG1, κ |
| TAB-1089CL | Human Anti-AXL Recombinant Antibody (TAB-1089CL) | ELISA, FuncS | Chimeric (mouse/human) IgG1, κ |
| TAB-1093CL | Mouse Anti-AXL Recombinant Antibody (TAB-1093CL) | ELISA, FC, Cyt, WB | |
| TAB-0138CL-F(E) | Mouse Anti-AXL Recombinant Antibody Fab Fragment (TAB-0138CL-S(P)) | ELISA, IF, FC, Inhib, ICFC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1090CL | Human Anti-AXL Recombinant Antibody (TAB-1090CL) | ELISA, FuncS | Chimeric (mouse/human) IgG1, κ |
| TAB-1091CL | Human Anti-AXL Recombinant Antibody (TAB-1091CL) | ADCC | Chimeric (mouse/human) IgG1, κ |
| TAB-1092CL | Human Anti-AXL Recombinant Antibody (TAB-1092CL) | ADCC | Chimeric (mouse/human) IgG1, κ |
| TAB-0189CL-F(E) | Human Anti-AXL Recombinant Antibody; Fab Fragment (TAB-0189CL-F(E)) | WB, FuncS, ELISA, FC | Chimeric (Mouse/Human) Fab |
| TAB-1090CL-F(E) | Human Anti-AXL Recombinant Antibody; Fab Fragment (TAB-1090CL-F(E)) | ELISA, FuncS | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1094CL | Anti-Human AXL Recombinant Antibody (Hz1613F12) | ELISA, FC, Cyt | Humanized antibody |
| TAB-1098CL | Anti-Human AXL Recombinant Antibody (Humanized 11B7) | ELISA, FuncS | Humanized antibody |
| TAB-1099CL | Anti-Human AXL Recombinant Antibody (Humanized 11D5) | ELISA | Humanized antibody |
| TAB-0139CL-F(E) | Anti-Human AXL Recombinant Antibody Fab Fragment (hzl613F12-24 DAR2) | ELISA, IF, FC, Inhib, ICFC | Humanized Antibody |
| TAB-0140CL-F(E) | Anti-Human AXL Recombinant Antibody Fab Fragment (hzl613F12-33 DAR4) | ELISA, IF, FC, Inhib, ICFC | Humanized Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0872MZ | Mouse Anti-AXL Recombinant Antibody (clone 1B3A2) | WB | Mouse IgG1 |
| HPAB-0600-CN | Human Anti-AXL Recombinant Antibody (HPAB-0600-CN) | SPR, ELISA, FC, WB, Inhib, FuncS, IHC, Block | Human IgG |
| HPAB-0601-CN | Human Anti-AXL Recombinant Antibody (HPAB-0601-CN) | ELISA, FC, IHC, Block | Human IgG |
| HPAB-0603-CN | Human Anti-AXL Recombinant Antibody (HPAB-0603-CN) | WB, ELISA, FC, IHC | Chimeric (Mouse/Human) IgG1, κ |
| HPAB-1783LY | Mouse Anti-AXL Recombinant Antibody (HPAB-1783LY) | ELISA, IHC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3970 | Hi-Affi™ Rabbit Anti-AXL Recombinant Antibody (clone SI30DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1850LY-F(E) | Mouse Anti-AXL Recombinant Antibody; Fab Fragment (HPAB-1850LY-F(E)) | ELISA, IA, FC, IHC, WB, IP | Mouse Fab |
| HPAB-1851LY-F(E) | Mouse Anti-AXL Recombinant Antibody; Fab Fragment (HPAB-1851LY-F(E)) | ELISA, IA, FC, IHC, WB, IP | Mouse Fab |
| HPAB-1852LY-F(E) | Mouse Anti-AXL Recombinant Antibody; Fab Fragment (HPAB-1852LY-F(E)) | ELISA, IA, FC, IHC, WB, IP | Mouse Fab |
| HPAB-1853LY-F(E) | Mouse Anti-AXL Recombinant Antibody; Fab Fragment (HPAB-1853LY-F(E)) | ELISA, IA, FC, IHC, WB, IP | Mouse Fab |
| HPAB-1854LY-F(E) | Mouse Anti-AXL Recombinant Antibody; Fab Fragment (HPAB-1854LY-F(E)) | ELISA, IA, FC, IHC, WB, IP | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1850LY-S(P) | Mouse Anti-AXL Recombinant Antibody; scFv Fragment (HPAB-1850LY-S(P)) | ELISA, IA, FC, IHC, WB, IP | Mouse scfv |
| HPAB-1851LY-S(P) | Mouse Anti-AXL Recombinant Antibody; scFv Fragment (HPAB-1851LY-S(P)) | ELISA, IA, FC, IHC, WB, IP | Mouse scfv |
| HPAB-1852LY-S(P) | Mouse Anti-AXL Recombinant Antibody; scFv Fragment (HPAB-1852LY-S(P)) | ELISA, IA, FC, IHC, WB, IP | Mouse scfv |
| HPAB-1853LY-S(P) | Mouse Anti-AXL Recombinant Antibody; scFv Fragment (HPAB-1853LY-S(P)) | ELISA, IA, FC, IHC, WB, IP | Mouse scfv |
| HPAB-1854LY-S(P) | Mouse Anti-AXL Recombinant Antibody; scFv Fragment (HPAB-1854LY-S(P)) | ELISA, IA, FC, IHC, WB, IP | Mouse scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY26 | AbPlus™ Anti-AXL Magnetic Beads (VS-0424-XY26) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY44 | Rabbit Anti-NHP AXL Recombinant Antibody (VS-1024-XY44) | WB, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY2 | CytoStream™ Rat Anti-AXL Recombinant Antibody (clone 175128) | FC | Rat IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY66 | Human Anti-AXL (clone Enapotamab) scFv-Fc Chimera | ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC363 | Recombinant Anti-AXL Vesicular Antibody, EV Displayed (VS-0425-YC363) | ELISA, FC, Inhib, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC20 | Recombinant Anti-AXL (AA 1-134 x AA 222-234) Biparatopic Antibody, Tandem scFv | Block, ELISA, FC, IHC, SPR, WB | Tandem scFv |
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