Human Anti-CD274 Recombinant Antibody (HPAB-1440LY)

Name: Human Anti-CD274 Recombinant Antibody (HPAB-1440LY)
SKU: HPAB-1440LY
Rating: 5 (1 reviews)

CAT#: HPAB-1440LY

This product is a chimeric (rabbit/human) antibody that recognizes CD274.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1899_B9_6_cr5ba2049576272_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line BJ

Immunofluorescent staining of human cell line BJ shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1936_G3_3_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3528/1953_G5_3_blue_red_green.jpg

Normal Tissue Figure 4 Colon

Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_9_3.jpg

Normal Tissue Figure 5 Placenta

Cytotrophoblasts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76385/169546_A_1_7.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-251 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120217-CD274

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Specifications

Host Species
Human

Type
Chimeric (Rabbit/Human) IgG1

Specificity
Human CD274

Species Reactivity
Human

Applications
IHC

Conjugate
None, customized conjugetion is available

Related Disease
Malignancies

Product Property

Purification
Protein A/G purified

Purity
>95% determined by SDS-PAGE (Reduced conditions), customized purity is available

Format
Liquid or lyophilized powder

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS, pH 7.4

Preservative
No preservatives

Storage
Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Applications

Application Notes
This antibody has been reported for use in Immunohistochemistry.

Target

Alternative Names
CD274 molecule; B7-H; B7H1; PDL1; PD-L1; hPD-L1; PDCD1L1; PDCD1LG1; programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1

Gene ID
29126

UniProt ID
Q9NZQ7

Protein Refseq
NP_001254635.1

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-1440LY.pdf

MSDS

MSDS - HPAB-1440LY.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

CAT
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Secondary Antibody

CAT
Product Name

Reconstitution Buffer

CAT
Product Name

Recommended Dilution Buffer

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See other products for "Cd274"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
TAB-0992CL	Mouse Anti-CD274 Recombinant Antibody (TAB-0992CL)	Inhib, FC, SPR	Mouse IgG
TAB-0262CL-F(E)	Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0262CL-F(E))	ELISA, WB, SPR, FuncS	Mouse Fab
TAB-1009CL-F(E)	Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1009CL-F(E))	WB, IHC	Mouse Fab
TAB-1010CL-F(E)	Anti-Human CD274 Recombinant Antibody Fab Fragment	FC
TAB-1011CL-F(E)	Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1011CL-F(E))	ELISA	Mouse Fab

CAT	Product Name	Application	Type
TAB-0995CL	Human Anti-CD274 Recombinant Antibody (TAB-0995CL)	ELISA, FC	Chimeric (Mouse/Human) IgG1, κ
TAB-0995CL-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (TAB-0995CL-S(P))	ELISA, FC	Human scFv
TAB-0995CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0995CL-F(E))	ELISA, FC	Chimeric (Mouse/Human) Fab

CAT	Product Name	Application	Type
TAB-0263CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0263CL-F(E))	ELISA, WB, SPR, FuncS	Humanized Fab
TAB-0264CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0264CL-F(E))	ELISA, WB, SPR, Inhib, FuncS	Humanized Fab
TAB-0265CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0265CL-F(E))	ELISA, WB, SPR, FuncS	Humanized Fab
TAB-0266CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0266CL-F(E))	ELISA, WB, SPR, FuncS	Humanized Fab
TAB-0267CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-0267CL-F(E))	ELISA, WB, SPR, FuncS	Humanized Fab

CAT	Product Name	Application	Type
TAB-1003CL-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (TAB-1003CL-F(E))	FC, ELISA	Human Fab
TAB-0998CL	Anti-Human CD274 Recombinant Antibody (BII61-62)	ELISA, FuncS	Human antibody
TAB-0999CL	Anti-Human CD274 Recombinant Antibody (B50-6)	FuncS	Human antibody
TAB-1000CL	Human Anti-CD274 Recombinant Antibody (TAB-1000CL)	Inhib, FC	Human IgG1
TAB-1001CL	Human Anti-CD274 Recombinant Antibody (TAB-1001CL)	ADCC, Block, FuncS	Human IgG

CAT	Product Name	Application	Type
NEUT-324CQ	Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3.C6)	IHC-P, FC, Neut, ICC	Mouse IgG2b, κ
NEUT-325CQ	Human Anti-CD274 Recombinant Antibody (clone CBLS-206)	Neut	Human IgG1, κ
NEUT-326CQ	Human Anti-CD274 Recombinant Antibody (clone CBLS-207)	ELISA, Neut	Human IgG1, κ
NEUT-327CQ	Mouse Anti-CD274 Recombinant Antibody (clone MIH1)	FC, FuncS, Neut, IHC, IF	Mouse IgG1, κ
NEUT-330CQ	Rat Anti-CD274 Recombinant Antibody (clone MIH5)	FC, FuncS, Neut, IHC-Fr, WB	Rat IgG2a, λ

CAT	Product Name	Application	Type
NEUT-328CQ	Rat Anti-CD274 Recombinant Antibody (VMC171)	Block, FC	Rat IgG2
NEUT-329CQ	Rat Anti-CD274 Recombinant Antibody (clone 10F.9G2)	FC, IF, Block	Rat IgG2b, κ
NEUT-331CQ	Mouse Anti-CD274 Recombinant Antibody (clone alphaPD-L1)	ELISA, Block	Mouse IgG1, λ
NEUT-332CQ	Rabbit Anti-CD274 Recombinant Antibody (clone CBL1009)	Block	Rabbit IgG

CAT	Product Name	Application	Type
MOR-0548	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone DS548AB)	ELISA	Rabbit IgG
MOR-4049	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI116DS)	ELISA	Rabbit IgG
MOR-4050	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI117DS)	ICC, IF, FC	Rabbit IgG
MOR-4051	Hi-Affi™ Rabbit Anti-CD274 Recombinant Antibody (clone SI118DS)	FC	Rabbit IgG

CAT	Product Name	Application	Type
HPAB-0193-YC-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0193-YC-S(P))	ELISA	Human scFv
HPAB-0194-YC-S(P)	Mouse Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0194-YC-S(P))	ELISA	Mouse scFv
HPAB-0195-YC-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0195-YC-S(P))	SPR	Humanized scFv
HPAB-0196-YC-S(P)	Human Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-0196-YC-S(P))	FC	Human scFv
HPAB-M0455-YC-S(P)	Mouse Anti-CD274 Recombinant Antibody; scFv Fragment (HPAB-M0455-YC-S(P))	ELISA	Mouse scFv

CAT	Product Name	Application	Type
HPAB-0193-YC-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0193-YC-F(E))	ELISA	Humanized Fab
HPAB-0194-YC-F(E)	Mouse Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0194-YC-F(E))	ELISA	Mouse Fab
HPAB-0195-YC-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0195-YC-F(E))	SPR	Humanized Fab
HPAB-0196-YC-F(E)	Human Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0196-YC-F(E))	FC	Human Fab
HPAB-0197-YC-F(E)	Rabbit Anti-CD274 Recombinant Antibody; Fab Fragment (HPAB-0197-YC-F(E))	ELISA, FC	Rabbit Fab

CAT	Product Name	Application	Type
HPAB-N0408-YC-VHH	Recombinant Humanized Anti-CD274 Single Domain Antibody (HPAB-N0408-YC-VHH)	ELISA, FC, BL	Human VHH
NBD-0065-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0065-YJ-VHH)	ELISA	Camelid VHH
NBD-0066-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0066-YJ-VHH)	ELISA, Inihb	Camelid VHH
NBD-0067-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0067-YJ-VHH)	ELISA, FC	Camelid VHH
NBD-0068-YJ-VHH	Camelid Anti-CD274 Recombinant Single Domain Antibody (NBD-0068-YJ-VHH)	ELISA, FC	Camelid VHH

CAT	Product Name	Application	Type
HPAB-0046LY	Human Anti-CD274 Recombinant Antibody (HPAB-0046LY)	WB	Human IgG4
HPAB-0561LY	Human Anti-CD274 Recombinant Antibody (HPAB-0561LY)	ELISA, Block	Chimeric (mouse/human) IgG1
HPAB-0562LY	Human Anti-CD274 Recombinant Antibody (HPAB-0562LY)	ELISA, Block	Chimeric (mouse/human) IgG1
HPAB-0563LY	Human Anti-CD274 Recombinant Antibody (HPAB-0563LY)	ELISA, Block	Chimeric (mouse/human) IgG1
HPAB-0564LY	Human Anti-CD274 Recombinant Antibody (HPAB-0564LY)	ELISA, Block	Chimeric (mouse/human) IgG1

CAT	Product Name	Application	Type
AFC-TAB-417CQ	Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-417CQ)	ELISA, IHC, FC, IP, IF, Inhib	ADCC enhanced antibody
AFC-TAB-418CQ	Afuco™ Anti-CD274 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-418CQ)	ELISA, IHC, FC, IP, IF, BL	ADCC enhanced antibody

CAT	Product Name	Application	Type
VS-0924-YC110	Mouse Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC110), CDC Enhanced	ELISA, WB, SPR, ADCC, CDC	Antibody hexamer
VS-0924-YC111	Human Anti-CD274 Recombinant Antibody Hexamer (VS-0924-YC111), CDC Enhanced	ELISA, WB, SPR, ADCC, CDC, Inhib	Antibody hexamer

CAT	Product Name	Application	Type
VS-1024-XY375	Mouse Anti-NHP CD274 Recombinant Antibody (clone 29E.2A3)	FC	Mouse IgG2b, kappa

CAT	Product Name	Application	Type
VS-0225-XY32	CytoStream™ Mouse Anti-CD274 Recombinant Antibody (clone 29E.2A3)	FC	Mouse IgG2b, kappa

CAT	Product Name	Application	Type
VS-0325-FY65	Human Anti-CD274 (clone MI4736) scFv-Fc Chimera	ELISA, Inhib	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0425-YC608	Recombinant Anti-CD274 Vesicular Antibody, EV Displayed (VS-0425-YC608)	Block, ELISA, FC, Cell-uptake

CAT	Product Name	Application
VS-0525-XY1152	Anti-CD274 Immunohistochemistry Kit	IHC
VS-0525-XY1153	Anti-Mouse CD274 Immunohistochemistry Kit	IHC
VS-0525-XY1154	Anti-Human CD274 Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-YC202	Recombinant Anti-CD274 Biparatopic Antibody, Tandem scFv	ELISA, IHC, FC, IP, IF	Tandem scFv

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