Mouse Anti-ERBB2 Recombinant Antibody (VS3-CJ362)
CAT#: VS3-CJ362
This product is a mouse antibody that recognizes ERBB2.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1060/169_D12_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1338/if_selected.jpg
Normal Tissue
Figure 3 High expression in esophagus
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1383/4682_B_5_1_val_selected.jpg
Normal Tissue
Figure 4 IHC staining of human rectum
Immunohistochemical staining of human rectum shows weak cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/ihc_selected.jpg
Normal Tissue
Figure 5 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/ihc_selected.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43/1054_A_7_5.jpg
Normal Tissue
Figure 7 Breast
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20416/45831_B_3_4.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (OE19, SK-BR-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141736-ERBB2
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Specifications
- Immunogen
- KLH-conjugated synthetic peptide encompassing a sequence of human HER2
- Host Species
- Mouse
- Specificity
- Human ERBB2
- Species Reactivity
- Human
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 30% Glycerol, 0.87% NaCl, 0.42% Potassium phosphate, pH7.3.
- Preservative
- 0.01% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:1000-1:2000), Immunohistochemistry (1:200-1:500).
Target
- Alternative Names
- HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340; TKR1; HER-2/neu
- Gene ID
- 2064
- UniProt ID
- P04626
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus
- Post Translation Modifications
- Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172, PubMed:33497358).
Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007).
Dephosphorylated by PTPN12 (PubMed:27134172).
- Protein Refseq
- NP_001005862.1; NP_001276865.1; NP_001276867.1
- Function
- Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
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Related Diseases
Bladder Cancer
Non-small Cell Lung Cancer
Related Signaling Pathways
ErbB Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-761 | Anti-ERBB2 Recombinant Antibody (Margetuximab) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
| TAB-049CT | Anti-Human HER2/neu Recombinant Antibody (ChA21) | IHC, Inhib, WB, Activ, FC | Chimeric antibody (mouse/human) |
| TAB-049CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (ChA21) | WB | Chimeric antibody (mouse/human) |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G022E | Anti-ERBB2 immunotoxin 4D5 (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G022D | Anti-ERBB2 immunotoxin 4D5 (scFv)-DT | Cytotoxicity assay, Function study | |
| AGTO-G022G | Anti-ERBB2 immunotoxin 4D5 (scFv)-Gel | Cytotoxicity assay, Function study | |
| AGTO-G022R | Anti-ERBB2 immunotoxin 4D5 (scFv)-RTA | Cytotoxicity assay, Function study | |
| AGTO-G022S | Anti-ERBB2 immunotoxin 4D5 (scFv)-Sap | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-085 | Human Anti-ERBB2 Recombinant Antibody (PFBL-085) | ELISA, WB, FuncS | Human Fab |
| PFBC-089 | Mouse Anti-ERBB2 Recombinant Antibody (clone A21); Fab Fragment | ELISA, WB, FC | Mouse Fab |
| PSBW-161 | Mouse Anti-ERBB2 Recombinant Antibody (clone chA21); scFv Fragment | FC, WB | Mouse scFv |
| HPAB-M0073-YC-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-M0073-YC-F(E)) | ELISA, FC | Human Fab |
| HPAB-M0074-YC-F(E) | Human Anti-ERBB2 Recombinant Antibody; Fab Fragment (HPAB-M0074-YC-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-066 | Recombinant Anti-HER2 VHH Single Domain Antibody (PNBL-066) | SPR | Llama VHH |
| PNBL-067 | Recombinant Anti-HER2 VHH Single Domain Antibody (PNBL-067) | SPR | Llama VHH |
| PNBL-069 | Recombinant Anti-HER2 VHH Single Domain Antibody (PNBL-069) | ELISA | Llama VHH |
| HPAB-0201-FY | Recombinant llama Anti-ERBB2 Antibody (HPAB-0201-FY) | E | llama VHH |
| HPAB-0202-FY | Recombinant llama Anti-ERBB2 Antibody (HPAB-0202-FY) | E | llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBW-161 | Mouse Anti-ERBB2 Recombinant Antibody (clone chA21); Fab Fragment | FC, WB | Mouse Fab |
| HPAB-0091-FY-S(P) | Human Anti-ERBB2 Recombinant Antibody (clone MIL12); scFv Fragment | ELISA | Humanized scFv |
| HPAB-0193-WJ-S(P) | Mouse Anti-ERBB2 Recombinant Antibody (clone 6H8); scFv Fragment | ELISA | Mouse scFv |
| HPAB-M0073-YC-S(P) | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (HPAB-M0073-YC-S(P)) | ELISA, FC | Human scFv |
| HPAB-M0074-YC-S(P) | Human Anti-ERBB2 Recombinant Antibody; scFv Fragment (HPAB-M0074-YC-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-033CT | Human Anti-ERBB2 Recombinant Antibody (TAB-033CT) | ELISA, WB | Human IgG1, κ |
| TAB-038CT | Human Anti-ERBB2 Recombinant Antibody (TAB-038CT) | FuncS, FC | Humanized IgG1 |
| TAB-039CT | Anti-Human HER2/neu Recombinant Antibody (h1E11) | WB | Humanized antibody |
| TAB-041CT | Human Anti-ERBB2 Recombinant Antibody (TAB-041CT) | SPR, ELISA, WB | Humanized antibody |
| TAB-468CQ | Anti-Human ERBB2 Recombinant Antibody (Timigutuzumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-040CT | Anti-Human HER2/neu Recombinant Antibody (C6.5) | ELISA, WB | Human antibody |
| TAB-042CT | Anti-Human HER2/neu Recombinant Antibody (CRX01) | WB | Human antibody |
| TAB-043CT | Human Anti-ERBB2 Recombinant Antibody (TAB-043CT) | ELISA, Inhibion | Human IgG1, κ |
| TAB-044CT | Anti-Human HER2/neu Recombinant Antibody (TAB-044CT) | FC, Inhib, IF, Cyt, WB, Apop | Human antibody |
| TAB-045CT | Anti-Human HER2/neu Recombinant Antibody (Erbicin) | ELISA, Inhibition | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-034CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (TAB-034CT-S(P)) | WB | |
| TAB-036CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (6B2) | ELISA, WB | |
| TAB-037CT-S(P) | Anti-Human HER2/neu Recombinant Antibody scFv Fragment (7C2/7F3) | ELISA, WB | |
| TAB-270CQ-F(E) | Mouse Anti-ERBB2 Recombinant Antibody; Fab Fragment (TAB-270CQ-F(E)) | WB, Inhib | Mouse Fab |
| PABX-082-S (P) | Recombinant Human Anti-HER2 Antibody scFv Fragment (Gr6) | WB, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-024LC | Recombinant Anti-Human ERBB2 Antibody (Fab glycosylation) | ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-145LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-146LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-147LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-148LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| Gly-149LC | Recombinant Anti-Human ERBB2 Antibody (Fc glycosylation) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-177LC | Recombinant Anti-Human ERBB2 Antibody (Non-glycosylated) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0195MZ | Chicken Anti-ERBB2 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-737CQ | Mouse Anti-ERBB2 Recombinant Antibody (clone 7.16.4) | Block, IP, IF, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1178 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone DS1178AB) | IHC-P, IHC-Fr | Rabbit IgG |
| MOR-4586 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH99DS) | WB | Rabbit IgG |
| MOR-4587 | Hi-Affi™ Rabbit Anti-ERBB2 Recombinant Antibody (clone TH100DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2808 | PE-A*02:01/Human ERBB2 (YMIMVKCWM) MHC Tetramer | FCM | |
| MHC-LC2809 | PE-A*02:01/Human ERBB2 (YMIMVKCWMI) MHC Tetramer | FCM | |
| MHC-LC2823 | APC-A*02:01/Human ERBB2 (ALCRWGLLL) MHC Tetramer | FCM | |
| MHC-CN0641 | PE-A*02:01/Human ERBB2 (LIAHNQVRQV) MHC Tetramer | FCM, IHC | |
| MHC-CN0642 | APC-A*02:01/Human ERBB2 (LIAHNQVRQV) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0010-FY | Human Anti-ERBB2 Recombinant Antibody (HPAB-0010-FY) | ELISA, FC, ADCC | Human IgG1, κ |
| HPAB-0011-FY | Mouse Anti-ERBB2 Recombinant Antibody (HPAB-0011-FY) | Inhib, ADCC, FuncS | Mouse IgG1, κ |
| HPAB-0733LY | Human Anti-ERBB2 Recombinant Antibody (clone AH06) | ELISA | Human IgG |
| HPAB-0734LY | Human Anti-ERBB2 Recombinant Antibody (clone A058) | ELISA | Human IgG |
| HPAB-0735LY | Human Anti-ERBB2 Recombinant Antibody (clone AH16) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-468CQ | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-468CQ) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-053 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-053) | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
| AFC-TAB-761 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-761) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-005 | Afuco™ Anti-ERBB2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-005) | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY91 | AbPlus™ Anti-ERBB2 Magnetic Beads (3.F2) | IP, Protein Purification | |
| VS-0724-YC1516 | AbPlus™ Anti-Erbb2 Magnetic Beads (VS-0724-YC1516) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY24 | Human Anti-ERBB2 (clone 1.18.1) scFv-Fc Chimera | FC, BI | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY108 | CytoStream™ Mouse Anti-ERBB2 Recombinant Antibody (clone 24D2) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC430 | Recombinant Anti-ERBB2 Vesicular Antibody, EV Displayed (VS-0425-YC430) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2335 | Anti-ERBB2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2336 | Anti-Mouse ERBB2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC3 | Recombinant Anti-ERBB2 Biparatopic Antibody, Symmetric scFv-Fab | ELISA | Symmetric scFv-Fab |
| VS-0525-YC6 | Recombinant Anti-ERBB2 Biparatopic Antibody, Asymmetric hetero Fab | ELISA | Asymmetric hetero Fab |
| VS-0525-YC7 | Recombinant Anti-ERBB2 Biparatopic Antibody, Asymmetric hetero HC-LC | ELISA, Inhib | Asymmetric hetero HC, common LC |
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