Mouse Anti-ERBB2 Recombinant Antibody (VS3-CJ363) (CAT#: VS3-CJ363)

• Immunogen
• KLH-conjugated synthetic peptide encompassing a sequence of human HER2

• Host Species
• Mouse

• Specificity
• Human, Mouse and Rat ERBB2

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, IHC

• Conjugate
• Unconjugated

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 30% Glycerol, 0.87% NaCl, 0.42% Potassium phosphate, pH7.3.

• Preservative
• 0.01% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Western Blot (1:2000-1:5000), Immunohistochemistry (1:50-1:200).

• Alternative Names
• HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340; TKR1; HER-2/neu

• Gene ID
• 2064

• UniProt ID
• P04626

• Sequence Similarities
• Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.

• Cellular Localization
• Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus

• Post Translation Modifications
• Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172, PubMed:33497358).
  Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007).
  Dephosphorylated by PTPN12 (PubMed:27134172).

• Protein Refseq
• NP_001005862.1; NP_001276865.1; NP_001276867.1

• Function
• Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
  In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

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