Genetic Engineering

With extensive experience in antibody engineering and recombinant monoclonal antibody manufacturing, Creative Biolabs now provides the custom genetic engineered bispecific antibody development service, enabling clients to adjust the format, size, valency, flexibility and half-life of bispecific antibody (BsAb) to achieve the desired BsAb profile.

Our experts are committed to offering the most suitable strategy to meet the needs of each customer. To design the specific BsAb, we use the elegant “knobs-into-holes” technique as well as the appropriate linkers and VH/VL domains orientation to promote the formation of BsAb molecules correctly; for expression of the desired BsAbs, a series of different vectors and expression systems (mammalian and microbial systems) are available.

To take the advantage of state-of-the-art facilities and the mature genetic engineering technology, Creative Biolabs has established a low-cost, high-yield and time-efficient BsAb development platform with the capability of generating multiple bispecific antibodies for both academic and industrial purposes.

Figure: Expression of human bispecific antibodies (Nat Biotechnol, 2014)

The main procedures for developing BsAbs by using genetic engineering include:

1. Molecular design, including the design of an optimal linker and VH/VL domains orientation
2. Stable cell lines construction, including the generation of high expression vectors and high-yield and stable cell lines
3. Scale-up production, purification and characterization

We offer a full range of techniques approaches needed for bispecific antibody development:

• Genetic Engineering
• Chemical Conjugation
• Hybrid–hybridoma Technology

Reference

1. Romain Rouet and Daniel Christ. Bispecific antibodies with native chain structure. Nat Biotechnol. 2014 Feb;32(2):136-7.

For research use only. Not intended for any clinical use.