Mouse Anti-MS4A1 Recombinant Antibody (VS-0724-FY35)

Name: Mouse Anti-MS4A1 Recombinant Antibody (VS-0724-FY35)
SKU: VS-0724-FY35
Rating: 5 (1 reviews)

CAT#: VS-0724-FY35

This product is a mouse IgG2b recombinant monoclonal antibody that binds to human and mouse CD20. The antibody HB20-3 binds to the B cell lines, Raji, BJAB, and DHL-4. HB20-3 also binds to primary B cells and B lymphoma cell lines that express significantly higher levels of CD20. The immunogen of HB20-3 is a mouse pre-B cell line transfected with cDNA encoding human CD20. The MB20-1 monoclonal antibody binds to cell surface CD20 at a higher density. This MB20-1 antibody was generated in CD20^-/- mice, which have a genetic background of C57BI/6 x 129 and have been immunized with the mouse pre-B cell line 300.19, which has been transfected with a cDNA encoding human CD20.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line REH

Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/1778_G3_5_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/201_H5_1_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/202_H5_1_red_green.jpg

Normal Tissue Figure 4 Colon

Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_3.jpg

Normal Tissue Figure 5 Bone marrow

Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_B_4_4.jpg

Normal Tissue Figure 6 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_8.jpg

RNA Expression Figure 7 RNA cell line category: Group enriched (Daudi, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156738-MS4A1

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Specifications

Immunogen
Mouse pre-B cell line transfected with human CD20 cDNAs

Host Species
Mouse

Derivation
Hybridoma

Specificity
Human, Mouse MS4A1

Species Reactivity
Human, Mouse

Applications
Flow Cytometry

Related Disease
Autoimmune disease

Product Property

Clonality
Monoclonal Antibody

Purity
>95% as determined by SDS-PAGE and SEC-HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Stabilizer
No Stabilizers

Sterility
0.2 μM filtered

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
Flow cytometry: 10 µg/mL

Target

Alternative Names
Membrane Spanning 4-Domains A1; Membrane-Spanning 4-Domains, Subfamily A, Member 1; Leukocyte Surface Antigen Leu-16; CD20 Antigen; CD20; Bp35; Membrane-Spanning 4-Domains Subfamily A Member 1; B-Lymphocyte Cell-Surface Antigen B1; B-Lymphocyte Surface Antigen B1

Gene ID
931

UniProt ID
P11836

Long Name
Membrane Spanning 4-Domains A1

Sequence Similarities
Belongs to the MS4A family.

Cellular Localization
Cell membrane, Membrane

Post Translation Modifications
Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate.

Protein Refseq
NP_068769.2; NP_690605.1

Function
B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes.
Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-0724-FY35.pdf

MSDS

MSDS - VS-0724-FY35.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Isotype Control

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Secondary Antibody

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