ScFv1-PEG-scFv2 is one of the popular bispecific antibody (BsAb) conjugate formats. Creative Biolabs can provide the production service of scFv1-PEG-scFv2 BsAb for our customers.
Figure: Addition of functionalities into the crosslinker
During the generation of BsAbs, it is possible to add new functionalities via cross-linkers. ScFv1-PEG-scFv2 BsAb is such a kind of BsAb that consists of two scFvs (single chain fragment variable) targeting two different antigens and a cross-linker containing PEG. Thus, this type of BsAb has three reactive groups, two scFv reactive groups and a PEG. The introduction of PEG into cross-linker has many advantages. Firstly, the risks of inactivating the antibody and the heterogeneity of the product are reduced due to its site-specific manner. Furthermore, it can extend the antibody’s half-life. Besides PEG, some other molecules can also be introduced such as antibody fragments, peptides, siRNA, toxic drugs or ﬂuorophores.
For research use only. Not intended for any clinical use.