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    Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-312MZ-S(P)) (CAT#: TAB-312MZ-S(P))

    This product is a recombinant mouse antibody scFv fragment that recognizes EGFR (Epidermal growth factor receptor, also known as ErbB-1, HER1). The epitope is in the EGFR Domain III. It binds cynomolgous EGFR. The antibody inhibits EGF binding and inhibits proliferation of cancer cell lines, A431 and HN-5. The antibody was purified by affinity chromatography.

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    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg
    Figure 2 IF staining of human cell line U-251 MG
    Figure 2 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg
    Normal Tissue
    Figure 3 Colon
    Figure 3 Colon
    (Glandular cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg
    Figure 4 Liver
    Figure 4 Liver
    (Cholangiocytes
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg
    Figure 5 Kidney
    Figure 5 Kidney
    (Bowman's capsule
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Collecting ducts
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Distal tubules
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Proximal tubules (cell body)
    Staining:Medium
    Intensity: Strong
    Quantity: <25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg
    Figure 6 Testis
    Figure 6 Testis
    (Leydig cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Pachytene spermatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Round or early spermatids
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg
    Figure 7 Placenta
    Figure 7 Placenta
    (Cytotrophoblasts
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Decidual cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Hofbauer cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Syncytiotrophoblasts - cell body
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Syncytiotrophoblasts - microvilli
    Staining:High
    Intensity: Strong)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Non-germinal center cells
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Cell line enriched (A-431)
    Figure 9 RNA cell line category: Cell line enriched (A-431)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

    Specifications

    • Immunogen
    • EGFR proteins
    • Host Species
    • Mouse
    • Type
    • Mouse scFv
    • Specificity
    • Human EGFR (Epidermal growth factor receptor, also known as ErbB-1, HER1)
    • Species Reactivity
    • Human, Monkey
    • Applications
    • Enzyme-linked Immunosorbent Assay
    • Related Disease
    • Cancer that is resistant to known anti-EGFR antibodies

    Product Property

    • Purity
    • >95% as determined by SDS-PAGE
    • Buffer
    • PBS
    • Preservative
    • No preservatives
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Target

    • Alternative Names
    • EGFR; epidermal growth factor receptor; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; erb-b2 receptor tyrosine kinase 1; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog

    Related Resources

    • Related Diseases
    • Related Signaling Pathways

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    CAT Product Name Application Type
    TAB-H35 Anti-Human EGFR Recombinant Antibody (Futuximab) IF, WB, Inhib IgG1 - kappa
    TAB-0225CL-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-0225CL-F(E)) Block, Inhib, FuncS, Apop, In vivo Chimeric (Mouse/Human) Fab
    TAB-272MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-272MZ-S(P)) FuncS Mouse scFv
    TAB-302MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-302MZ-S(P)) ELISA Mouse scFv
    TAB-308MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-308MZ-S(P)) ELISA, FC Mouse scFv

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