Recombinant Humanized (from rat) antibody to Human CD3E. Otelixizumab, also known as TRX4, is a monoclonal antibody, which is being developed for the treatment of type 1 diabetes and other autoimmune diseases.
Figure 1 TGF-b is required for the effects of otelixizumab treatment in NOD-huCD3e mice.
Treatment with a neutralizing antibody to TGF-b (2G.7) inhibits otelixizumab-induced remission of diabetes inNOD-huCD3e mice(black squares). Anti–TGF-b antibody was administered intraperitoneally (1 mg per injection) on days 1 to 6 after the first otelixizumab administration.
Kuhn, C. , You, S. , Valette, F. , Hale, G. , Van Endert, P. , & Bach, J. F. , et al. (2011). Human cd3 transgenic mice: preclinical testing of antibodies promoting immune tolerance. Science Translational Medicine, 3(68), 68-68.
Figure 2 TGF-b is required for the effects of otelixizumab treatment in NOD-huCD3e mice.
Inhibitory effect of recombinant TGF-b (rTGF-b) on in vitro anti-CD3–induced proliferation of CD4+ CD25− T cells from 6-week-old (black circles), diabetic (black squares), or otelixizumab-treated (gray triangles) NOD-huCD3e mice. Data represent mean ± SEM values from three independent experiments. Asterisks indicate significant differences in mean values: **P < 0.01, ***P < 0.001.
Kuhn, C. , You, S. , Valette, F. , Hale, G. , Van Endert, P. , & Bach, J. F. , et al. (2011). Human cd3 transgenic mice: preclinical testing of antibodies promoting immune tolerance. Science Translational Medicine, 3(68), 68-68.
Figure 3 T cells from NOD-huCD3e mice respond to both anti-human and antimouse CD3 antibody stimulation.
Apoptotic T cells (7-AAD−annexin V+)(mean ± SEM) as assessed byflow cytometry within splenic CD4+ and CD8+ T cell compartments after a single infection of mitogenic or nonmitogenic anti-mouse [2C11 or F(ab′)2 fragments of 2C11] or anti-human (YTH12.5 or otelixizumab) CD3 antibodies. Results presented are representative of experiments performed on six to eight mice or individual human samples.
Kuhn, C. , You, S. , Valette, F. , Hale, G. , Van Endert, P. , & Bach, J. F. , et al. (2011). Human cd3 transgenic mice: preclinical testing of antibodies promoting immune tolerance. Science Translational Medicine, 3(68), 68-68.
Figure 4 Schematic representation of the humanization strategy for CD3E, CD3D, and CD3G.
Representative results of RT-PCR analysis for mouse Cd3 edg or human CD3 EDG expression in the blood cells.
Ueda, O. , Wada, N. A. , Kinoshita, Y. , Hino, H. , Kakefuda, M. , & Ito, T. , et al. (2018). Corrigendum: entire cd3ε, δ, and γ humanized mouse to evaluate human cd3–mediated therapeutics. Scientific Reports, 8, 46960.
Figure 5 Immunohistochemical staining for CD3 in (A) the thymus and (B) the spleen.
The T cell zone in each tissue was specifically stained for CD3 in human CD3 EDG–replaced mice and in wild-type mice. No staining was detected in the tissues of Cd3 edg−/− mice. wt, wild-type mice; mCd3 edg−/−, mouse Cd3 edg knockout mice; hCD3E Tg, human CD3E transgenic mice; 4HH3, 8I12 and 1C3 indicate the three different strains of human CD3 EDG–replaced mice; M, medulla; C, cortex; p< periarteriolar lymphoid sheaths (PALS); F, follicles; R, red pulps; In mCd3 edg−/−, the boundary between cortex (C) and medulla (M) was not clear in either the thymus or the spleen.
Ueda, O. , Wada, N. A. , Kinoshita, Y. , Hino, H. , Kakefuda, M. , & Ito, T. , et al. (2018). Corrigendum: entire cd3ε, δ, and γ humanized mouse to evaluate human cd3–mediated therapeutics. Scientific Reports, 8, 46960.
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• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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Afuco™ Anti-CD3E ADCC Recombinant Antibody (Otelixizumab), ADCC EnhancedThis product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant Humanized (from rat) antibody to Human CD3E. Otelixizumab, also known as TRX4, is a monoclonal antibody, which is being developed for the treatment of type 1 diabetes and other autoimmune diseases.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-H28 | Anti-Human CD3E Recombinant Antibody (Ertumaxomab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG2a / G2b |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-714 | Anti-CD3E Recombinant Antibody (Visilizumab) | FuncS, IF, Neut, ELISA, FC, IP, ICC | IgG2 |
TAB-420CQ | Anti-Human CD3E Recombinant Antibody (Visilizumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG2, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-245 | Anti-Human CD3 epsilon Recombinant Antibody (Foralumab) | WB, ELISA, FC, IP, FuncS, IF, Neut | IgG1 - kappa |
TAB-124CL | Human Anti-CD3E Recombinant Antibody (TAB-124CL) | ELISA | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-019 | Anti-Human CD3 Recombinant Antibody (Muromonab-CD3) | FC, IP, ELISA, Neut, FuncS, IF, WB | IgG2a - kappa |
PABX-032-S (P) | Recombinant Grey hamster Anti-CD3E Antibody scFv Fragment (2C11) | WB, ELISA, FuncS | scFv |
PABX-033-S (P) | Recombinant Grey hamster Anti-CD3E Antibody scFv Fragment (17A2) | WB, ELISA, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
AGTO-G010E | Anti-CD3E immunotoxin UCHT1 (scFv)-PE | Cytotoxicity assay, Function study | |
AGTO-G010D | Anti-CD3E immunotoxin UCHT1 (scFv)-DT | Cytotoxicity assay, Function study | |
AGTO-G010R | Anti-CD3E immunotoxin UCHT1 (scFv)-RTA | Cytotoxicity assay, Function study | |
AGTO-L049E | anti-CD3E immunotoxin UCHT1 (IgG)-PE | Cytotoxicity assay, Functional assay | |
AGTO-L049D | anti-CD3E immunotoxin UCHT1 (IgG)-DT | Cytotoxicity assay, Functional assay |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-643 | Mouse Anti-CD3E Recombinant Antibody (clone 2C11); Fab Fragment | WB, IHC, IF, FuncS | Mouse Fab |
FAMAB-0049-YC-F(E) | Hamster Anti-CD3E Recombinant Antibody (clone 145-2C11); Fab Fragment | FC, FuncS | Hamster Fab |
FAMAB-0052-YC-F(E) | Hamster Anti-CD3E Recombinant Antibody (clone 500A2); Fab Fragment | ELISA | Hamster Fab |
FAMAB-0047CQ-F(E) | Mouse Anti-CD3E Recombinant Antibody (clone CLB-T3/4.1); Fab Fragment | ELISA, IF, IP | Mouse Fab |
HPAB-0263-YJ-F(E) | Rabbit Anti-CD3E Recombinant Antibody (clone 645); Fab Fragment | ELISA, FC, FuncS | Rabbit Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-643 | Mouse Anti-CD3E Recombinant Antibody (clone 2C11); scFv Fragment | WB, IHC, IF, FuncS | Mouse scFv |
FAMAB-0049-YC-S(P) | Hamster Anti-CD3E Recombinant Antibody (clone 145-2C11); scFv Fragment | FC, FuncS | Hamster scFv |
FAMAB-0052-YC-S(P) | Hamster Anti-CD3E Recombinant Antibody (clone 500A2); scFv Fragment | ELISA | Hamster scFv |
FAMAB-0047CQ-S(P) | Mouse Anti-CD3E Recombinant Antibody (clone CLB-T3/4.1); scFv Fragment | ELISA, IF, IP | Mouse scFv |
HPAB-0385-FY-S(P) | Human Anti-CD3E Recombinant Antibody; scFv Fragment (HPAB-0385-FY-S(P)) | Activ | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-032 | Recombinant Grey hamster Anti-CD3E Antibody (2C11) | WB, ELISA, FuncS, IP | IgG |
PABX-033 | Recombinant Grey hamster Anti-CD3E Antibody (17A2) | WB, ELISA, FuncS | IgG |
PABX-032-F (E) | Recombinant Grey hamster Anti-CD3E Antibody Fab Fragment (2C11) | WB, ELISA, FuncS | Fab |
PABX-033-F (E) | Recombinant Grey hamster Anti-CD3E Antibody Fab Fragment (17A2) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0558 | Hi-Affi™ Rabbit Anti-CD3E Recombinant Antibody (clone DS558AB) | IHC-P | Rabbit IgG |
MOR-0058-FY | Rabbit Anti-CD3E Recombinant Antibody (clone AFY0029) | FC, ICC, IF, IHC-P, WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-245 | Afuco™ Anti-CD3E ADCC Recombinant Antibody (Foralumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF | ADCC enhanced antibody |
AFC-TAB-H52 | Afuco™ Anti-CD3E ADCC Recombinant Antibody (Otelixizumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
AFC-TAB-714 | Afuco™ Anti-CD3E ADCC Recombinant Antibody (Visilizumab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
AFC-TAB-019 | Afuco™ Anti-CD3E ADCC Recombinant Antibody (Muromonab-Cd3), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
FN-098CQ | Mouse Anti-CD3E Recombinant Antibody (clone HIT3a) | FuncS | Mouse IgG2a, κ |
HPAB-0347-YJ | Rat Anti-CD3E Recombinant Antibody (clone 1Ab) | FC, WB, Inhib, FuncS | Rat IgG |
ZG-0941F | Mouse Anti-CD3E Recombinant Antibody (ZG-0941F) | FC | Mouse IgG |
ZG-0945F | Mouse Anti-CD3E Recombinant Antibody (ZG-0945F) | FC | Mouse IgG |
VS4-WK82 | Mouse Anti-CD3E Recombinant Antibody (VS4-WK82) | ELISA, IF, FC | Mouse IgG2a |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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