Magic™ Phaeodactylum tricornutum Expression Service

Developing the most advanced algae expression system, the Magic™ Phaeodactylum tricornutum Expression Service of Creative Biolabs adopted the cutting-edge technique with great diligent efforts for optimal efficiency by numerous creative strategies and experimental trials. Accumulating along the irreversible time, our service has won the huge challenge of producing complex proteins (majority of antibodies, relatively large enzyme with complicated structure, etc.) with a demanding purity and large scale at relatively low expenditure. Since the wisdom nature has given us the incredible algae Phaeodactylum tricornutum as a beautiful gift to produce superior life-saving biopharmaceuticals, the Magic™ Phaeodactylum tricornutum Expression Service is proud of introducing it to the basic research and clinical applications of the whole world.

The Introduction and Application of Phaeodactylum tricornutum Expression

Recognized as a diatom, the P. tricornutum is a widely spread micro-algae existing in morphotypes of oval, triradial and fusiform. Since it manifests several great merits for various biotechnological applications, such as the biofuel production, food industry, bioplastic usage, etc., this incredible organism has appealed so many interests of researchers to sequence its entire genome, establish and further optimize the transfection techniques, and the most suitable cultivation parameters. Thus, our creative scientists have developed it as the superior host to produce almost all kinds of biopharmaceuticals. The Magic™ Phaeodactylum tricornutum Expression Service possesses a sophisticated and powerful toolbox for the primary selection of expression vectors, plenty of feasible promoters, screen markers, a number of reporters, and a signal peptide library.

Advantages of Magic™ Phaeodactylum tricornutum Expression Service

1. Excellent secretory or subcellular accumulation by our optimized heterogenous expression system.
2. Systematic library and toolbox for easy manipulation and modification.
3. Optimal cultivation and induction with the whole range of scales from 5 mL to 1000 L per batch.
4. One-stop service: from initial design and optimization all the way down to purification and further modification with well recorded data and organized report.

Notably, we highly recommend the light-inducible promoter fcpB to take the critical responsibility for constitutive activation with a proper spectrum of light. Basically, the fcpB promoter controls the expression of fucoxanthin binding proteins, which functions to shield the damage of light-harvesting complex (LHC) from an intensive sunlight in natural algae. Another amazing characteristic of P. tricornutum is its excellent secretory ability, which simply secrets the heterologous proteins into the extremely simple medium. No matter the protein accumulation or the subsequent downstream purification, it greatly elevates the accumulation amount and simplifies the purification procedure resulting in more yield and purity. Winging up with a membrane targeting sequences (MTSs), the target protein spontaneously prefers to bind to the peripheral membrane, which further elevates the efficiency of secretion and yield. Moreover, since the microalgae secrets only a few biomolecules into the medium, the component contamination is extremely low, which greatly benefits the purification and valuable purity of final target.

At Creative Biolabs, our service has successfully produced multiple antibodies, hormones, and enzymes for varied purposes with the best dedication and enthusiasm. The highest destiny of our service is to offer the most superior bioproduct in the world. If you have any question, contact us now.

References

1. Hempel, Franziska, et al. "Algae as protein factories: expression of a human antibody and the respective antigen in the diatom Phaeodactylum tricornutum." PloS one 6.12 (2011): e28424.
2. Veith, Thomas, and Claudia Büchel. "The monomeric photosystem I-complex of the diatom Phaeodactylum tricornutum binds specific fucoxanthin chlorophyll proteins (FCPs) as light-harvesting complexes." Biochimica et Biophysica Acta (BBA)-Bioenergetics 1767.12 (2007): 1428-1435.

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For research use only. Not intended for any clinical use.