Mouse Anti-CD4 Recombinant Antibody (VS3-CJ268) (CAT#: VS3-CJ268)

• Immunogen
• Synthetic peptide

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human, Mouse and Rat CD4

• Species Reactivity
• Human, Mouse, Rat

• Applications
• ICC

• Conjugate
• Unconjugated

• Purification
• Affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• 50% Glycerol, PBS, pH7.4.

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Immunocytochemistry (1:50-1:200), Immunohistochemistry (1:200).

• Alternative Names
• T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD_antigen: CD4; CD4mut

• Gene ID
• 920

• UniProt ID
• P01730

• Cellular Localization
• Cell membrane, Membrane

• Post Translation Modifications
• Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  Phosphorylated by PKC; phosphorylation at Ser-433 plays an important role for CD4 internalization.

• Protein Refseq
• NP_000607.1

• Function
• Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
  (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed:2214026, PubMed:16331979, PubMed:9641677, PubMed:12089508).
  Down-regulated by HIV-1 Vpu (PubMed:17346169).
  Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed:7909607).

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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