Mouse Anti-MS4A1 Monoclonal Antibody (VS7-0425-WR415)

Name: Mouse Anti-MS4A1 Monoclonal Antibody (VS7-0425-WR415)
SKU: VS7-0425-WR415
Rating: 5 (1 reviews)

CAT#: VS7-0425-WR415

This product is a mouse IgG antibody that binds to human MS4A1. The antibody recognizes specific amino acid domain of human MS4A1 (aa (EPANPSEKNSPSTQY)). It can be used for IHC-P, IF, ICC, and ELISA assays. It cross reacts with mouse.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line REH

Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/1778_G3_5_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/201_H5_1_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/202_H5_1_red_green.jpg

Normal Tissue Figure 4 Colon

Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_3.jpg

Normal Tissue Figure 5 Bone marrow

Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_B_4_4.jpg

Normal Tissue Figure 6 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_8.jpg

RNA Expression Figure 7 RNA cell line category: Group enriched (Daudi, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156738-MS4A1

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Specifications

Immunogen
Recombinant human MS4A1 (aa (EPANPSEKNSPSTQY)) fragment expressed in E. Coli.

Host Species
Mouse

Type
Mouse IgG

Specificity
Human MS4A1

Species Reactivity
Human

Molecule Class
B-Lymphocyte Surface Molecule

Applications
IHC-P, IF, ICC, ELISA

Conjugate
Unconjugated

Related Disease
Immunodeficiency, common variable, 5 (CVID5)

Product Property

Clonality
Monoclonal

Purity
>95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid

Format
Liquid

Concentration
1 mg/ml

Preservative
0.03% sodium azide

Storage
Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
Immunohistochemistry: 1:200 - 1:1000
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000

Target

Alternative Names
MS4A1;membrane-spanning 4-domains, subfamily A, member 1;CD20;B-lymphocyte antigen CD20;B1;Bp35;CD20 antigen;CD20 receptor;leukocyte surface antigen Leu-16;B-lymphocyte cell-surface antigen B

Gene ID
931

UniProt ID
P11836

Long Name
Membrane Spanning 4-Domains A1

Cellular Localization
Cell membrane; Membrane

Post Translation Modifications
Disulfide bond; Lipoprotein; Palmitate; Phosphoprotein

Protein Refseq
NP_068769.2

Function
B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:12920111, PubMed:3925015, PubMed:7684739).; Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:12920111, PubMed:18474602, PubMed:7684739).

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS7-0425-WR415.pdf

MSDS

MSDS - VS7-0425-WR415.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

CAT
Product Name

Secondary Antibody

CAT
Product Name

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Gly-138LC	Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated)	ELISA	Humanized antibody
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Gly-158LC	Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated)	ELISA	Chimeric antibody (mouse/human)

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Gly-142LC-1	Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Hyper-galactosylated)	ELISA	Human antibody

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