Tissue culture is a biological research method in which plant cells, tissues or organs are cultured on specially formulated nutrient media. This technique transfers tissue fragments from animals or plants into an artificial environment where they can continue to survive and function. Due to its wide range of applications, tissue culture is very important in biology.
Both plant and animal tissues can be used in culture. Creative Biolabs offers our clients the service of tissue culture according to optimized protocols. We use animal tissue culture to help preserve organs or tissues, and we use plant tissue culture for genetic modification or other functions in plants.
|Stages||Solutions and Reagents|
|Preparation||Culture medium, tissue materials, antibiotic, distilled water, and washing buffer|
Figure 1: Tissue Culture Procedure.
The medium used for culture can be broth or agar. There are two options for preparing the medium. One is to purchase all the chemicals in the culture medium and prepare it yourself as needed. The second is to purchase a commercially well-mixed medium. The exact amount used here will depend on the amount of tissue to be cultured. Heat and stir to dissolve, then pour the warm medium into the tube. Cover and place the tube in a pressure cooker, and sterilize.
Sterilization is one of the most important tasks in tissue culture. After preparing the medium, all of the medium is autoclaved, along with any tools and containers that may be used.
Get the culture material ready. Cut plant or animal parts into small pieces, then sterilize and wash them. Transfer the plant material to sterile tissue culture medium on a sterile operating table. Cover tightly.
Place the culture material in the culture room and set up a suitable environment with light, temperature, and sterility to allow it to grow.
Poor tissue growth
The information given above lists some potential problems and provides possible solutions that can help you troubleshoot your tissue culture experiments. If you need more related services and information, please contact us.
For research use only. Not intended for any clinical use.