Turbidimetry is an analytical technique. It is a light scattering measurement method that measures the intensity of light to determine the concentration of a suspended substance. Turbidimetry can be used in biology to determine the number of cells in a solution and the concentration of antigen in the solution.
Creative Biolabs provides scientists and experimenters with first-class laboratory reagents, technologies, and services for turbidimetry. Here below, you will find professional, scientific test protocols and troubleshooting guides for your reference.
|Solutions and Reagents
|Distilled water, antigen standards, particle-bound antibodies, and reaction buffer
Turbidimetry is a highly sensitive assay that gives results in a short period of time. The method is commonly used to quantify antigen-antibody complexes. The formation of antigen-antibody complexes increases the turbidity of the sample, which is measured by observing the transmission level of visible light.
Figure 1: Turbidimetry Procedure.
Prepare multiple test tubes with distilled water, serial dilutions of antigen standards, and samples to be tested. Set them up as a blank group, a control group, and a sample group. Then add an equal amount of specific antibody reagent to each tube, mix well, and incubate.
In the turbidimetric assay, the particle-bound antibody forms turbidity with the sample in an appropriate buffer. After that, use a light source to analyze the mixture. The level of transmitted light reflects the amount of antigen in the solution. If no antigen is present, little or no light is absorbed. As the concentration of antigen increases, the light transmission decreases accordingly.
Read the absorbance of the standard set of samples at the set wavelength. Plot the standard curve between sample concentration and absorbance. Finally, read the absorbance of each sample tube to be measured and substitute into the standard curve for antigen concentration calculation.
We describe proper measurement methods and related troubleshooting tips that are expected to help you collect the best possible test data.
Too high/low detection values
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For research use only. Not intended for any clinical use.